Theanine Biosynthesis during Tea Seed Germination

Abstract

Theanine content was measured in tea (Camellia sinensis L.) seed during different stages of germination and seedling development. Theanine content increased from 0.1% to 2% during germination. Theanine content progressively increased till 60 days of germination and decreased thereafter. Theanine analysis in different organs revealed that the emerging radicle and plumule contained 10-12% and 8-10%) of theanine, respectively. Theanine content in the cotyledon decreased progressively over time during seedling development. The increase in theanine content during germination was found to be associated with increased theanine synthetase gene expression. Further, application of gibberellic acid during germination lead to dramatic increase in theanine content.