Differentiation and identificaiton of tea clones using RAPD and ISSR markers

  • Janhvi Mishra-Rawat Botany Division, Forest Research Institute, Dehradun, Uttarakhand, India.
  • Shymal K. Nandi G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, India
  • Anil Prakash Department of Biotechnology, Barkatuilah University, Bhopal, Madhya Pradesh, India.
  • Lok Man S. Palni G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, India

Abstract

The genetic differentiation and clonal identification of eight tea [Camellia sinensis (L.) O. Kuntze] clones selected from different agroclimatic zones of India were investigated in the present study using morphological and molecular markers (RAPD; Random Amplified Polymorphic DNA and ISSR; Inter Simple Sequence Repeats). Major morphological parameters (leaf area, leaf length, average leaf width and plant height) were considered and based on cluster analysis of leaf area two different groups were formed; clones BSS-449 and BSS-379 formed one group and remaining clones formed another. RAPD analysis produced 82.14% polymorphism among the clones. Two main groups were recognized from cluster analysis, clones UPASI-9 and T-383 formed one cluster and the remaining clones another; similarity within the clones ranged from 0.33 to 0.91. ISSR data also revealed a pattern similar to that of RAPD markers. Maximum similarity was observed between the clones P-312 and AV-2. Although ISSR similarity matrix analysis showed two different clusters these markers were able to separate the Assam hybrid BSS-449 and it formed another clusters with clone BSS-379. ISSRs were found to be more discriminative in cultivar identification than RAPDs since clones that could not be distinguished by RAPD markers were easily differentiated by the ISSR markers. The results of this study may serve as basic information for rapid and easy characterization and subsequent identification of tea clones.
Keywords: Camellia sinensis, Characterization; ISSR marker; RAPD marker

Downloads

Statistics
633 Views | 463 Downloads
How to Cite
Mishra-Rawat, J., S. Nandi, A. Prakash, and L. M. Palni. “Differentiation and Identificaiton of Tea Clones Using RAPD and ISSR Markers”. International Journal of Tea Science, Vol. 8, no. 04, Dec. 2012, pp. 36-43, doi:10.20425/ijts.v8i4.4710.