Tea tissue culture: In vitro shoot regeneration and callus induction in tea

  • Usha Mukundan Plant Biotechnology Laboratory, Ramniranjan Jhunjhunwala College, Ghatkopar, Mumbai, India
  • Jayesh Patil Plant Biotechnology Laboratory, Ramniranjan Jhunjhunwala College, Ghatkopar, Mumbai, India
  • Mugdha Ambatkar Plant Biotechnology Laboratory, Ramniranjan Jhunjhunwala College, Ghatkopar, Mumbai, India
  • Sreeramulu Guttapadu Hindustan Unilever Research Centre, 64, Whitefield Main Road, Bengaluru, India
  • Vilas Sinkar Hindustan Unilever Research Centre, 64, Whitefield Main Road, Bengaluru, India
  • R. P. Karunagoda Department of Agricultural Biology, University ofPeradeniya, Peradeniya, Sri Lanka

Abstract

Camellia sinensis, or Tea, is one of the most important crop plants in the world. Biotechnological interventions
in the propagation of tea have become necessary as conventional methods prove insufficient. From the present study, MS
medium supplemented with 0.5 mg L"' Kinetin was found to be effective in inducing in vitro shooting from apical and nodal
explants derived from 3-month-old seedlings of Camellia sinensis cv. UPASI-9. For callus induction in in vitro grown leaves,
MS medium supplemented with 0.5 mg L"' NAA and 0.5 mg L"' 2,4-D was found to be most effective.
Keywords: Camellia sinensis; In vitro; Propagation; Callus

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How to Cite
Mukundan, U., J. Patil, M. Ambatkar, S. Guttapadu, V. Sinkar, and R. Karunagoda. “Tea Tissue Culture: In Vitro Shoot Regeneration And callus Induction in Tea”. International Journal of Tea Science, Vol. 9, no. 04, Dec. 2013, pp. 03-05, doi:10.20425/ijts.v9i4.4748.